paper 150205165314

Abstract

Previously we showed an interesting effectiveness of the electromagnetic informa- tion delivery of a specific source molecule in mimicking its specific biological effect. Nevertheless the effect was quite lower than the original positive control. Therefore we decide to assess a pos- sible synergism between a reduced dose of a molecule and its own electromagnetic information in order to increase the efficacy of this procedure trying to disclose new possible avenues in transla- tional pharmacology following suggestion from other works reporting synergistic effect between molecules and extremely low frequency electromagnetic fields. A reduced dose of retinoic acid, corresponding to a tenth of the usual, was delivered together with its own electromagnetic infor- mation to LAN-5 neuroblatoma cells. The signals from retinoic acid molecules was transferred to the cell culture medium employing as for our previous reports a commercial available electro medical device (Vegaselect 719). The effect on cell differentiation was significantly higher, and statistically significant, than the one obtained by the electromagnetic information delivery proce- dure when performed alone. A positive and effective synergism between a reduced drug dose and its own electromagnetic information seems to emerge as a promising and useful perspective in translational pharmacology outlining future applications in new drug delivery protocols allowing to reduce the amount of drug’s doses especially in the elderly and in the increasing number of patients with multiple comorbidities. Bioelectromagnetic and biochemistry should, therefore, be considered on the way of a promising wedding instead of a permanently independent life. Future researches should either optimize the protocols of preparation of these potentially new drugs, either assess the lifespan of their biological effect in order to translate them into effective clinical application at the bedside through Biolectromagnetic medicine.

1. Jerman, I., R. Ruźic, R. Krašovec, M. Škarja, and L. Mogilnicki, "Electrical transfer of molecule information into water, its storage, and bioeffects on plants and bacteria," Electromagn. Biol. Med., Vol. 24, No. 3, 341-353, 2005.
doi:10.1080/15368370500381620 Google Scholar

2. Jerman, I., M. Berden, and R. Ruźic, "Biological influence of ultraweak supposedly EM radiation from organisms mediated through water," Electro. and Magnetobiology, Vol. 15, No. 3, 229-244, 1996.
doi:10.3109/15368379609012879 Google Scholar

3. Endler, P. C., M. Citro, W. Pongratz, C. W. Smith, and C. Vinattieri, "Transfer of molecular information using a bioresonance instrument (BICOM) in amphibian trials," Acta Medica Empirica, Vol. 44, No. 3, 1-16, 1995. Google Scholar

4. Kreisl, P., "Tests on the transduction of acetic acid information via an electronic amplifier," Acta Medica Empirica, Vol. 47, 17-24, 1998. Google Scholar

5. Thomas, Y., M. Schiff, L. Belkadi, P. Jurgens, L. Kahhak, and J. Benveniste, "Activation of human neutrophils by electronically transmitted phorbol-myristate acetate," Med. Hypotheses, Vol. 54, 33-39, 2000.
doi:10.1054/mehy.1999.0891 Google Scholar

6. Calzoni, G. L., F. Borghini, E. Del Giudice, L. Betti, F. Dal Rio, M. Migliori, G. Trebbi, and A. Speranza, "Weak extremely high frequency microwaves affect pollen-tube emergence and growth in kiwifruit: Pollen grain irradiation and water-mediated effects," J. Altern Complement Med., Vol. 9, No. 2, 217-233, 2003.
doi:10.1089/10755530360623338 Google Scholar

7. Foletti, A., P. Baron, E. Sclauzero, G. Bucci, and A. Rinaudo, "Assessment of biophysical therapy in the management of pain in current medical practice compared with ibuprofen and placebo: A pilot study," J. Biol. Regul. Homeostat Agents, Vol. 28, No. 3, 431-439, 2014. Google Scholar

8. Foletti, A. and M. Cozzolino, "A biophysical integrated approach to autoimmune nephrotic syndrome: Case report," Recenti. Prog. Med., Vol. 104, No. 9, 488-489, 2013. Google Scholar

9. Baron, P., G. Bucci, A. Rinaudo, R. Rocco, E. Sclauzero, and A. Foletti, "System information therapy in the management of pain: A pilot study," PIERS Proceedings, 1698-1702, Stockholm, Sweden, August 12-15, 2013. Google Scholar

10. Smith, C. W., "Quanta and coherence effects in water and living systems," J. Altern Complement Med., Vol. 10, No. 1, 69-78, 2004.
doi:10.1089/107555304322848977 Google Scholar

11. Herrmann, E. and M. Galle, "Retrospective surgery study of the therapeutic effectiveness of the MORA bioresonance therapy with conventional therapy resistant patients suffering from allergies, pain and infection diseases," Eur. J. Integrative Med., Vol. 2, No. 4, 257-258, 2010.
doi:10.1016/j.eujim.2010.09.180 Google Scholar

12. Foletti, A. and J. Pokorny, "Biophysical approach to low back pain: A pilot report," Electromagn. Biol. Med., 2015, in press.
doi:10.3109/15368378.2015.1036075 Google Scholar

13. Smith, C. W., "Quanta and coherence effects in water and living systems," J. Altern Complement Med., Vol. 10, No. 1, 69-78, 2004.
doi:10.1089/107555304322848977 Google Scholar

14. Foletti, A., M. Ledda, E. D'Emilia, S. Grimaldi, and A. Lisi, "Differentiation of human LAN5 neuroblastoma cells induced by extremely low frequency electronically transmitted retinoic acid," J. Altern Complement Med., Vol. 17, No. 8, 701-704, 2011. Google Scholar

15. Foletti, A., M. Ledda, E. D'Emilia, S. Grimaldi, and A. Lisi, "Experimental finding on the electromagnetic information transfer of specific molecular signals mediated through the aqueous system on two human cellular models," J. Altern Complement Med., Vol. 18, No. 3, 258-261, 2012.
doi:10.1089/acm.2011.0104 Google Scholar

16. Foletti, A., M. Ledda, S. Piccirillo, S. Grimaldi, and A. Lisi, "Electromagnetic information delivery as a new tool in translational medicine," Int. J. Clin. Exp. Med., Vol. 7, No. 9, 2550-2556, 2014.
doi:10.1080/15368378.2017.1347882 Google Scholar

17. Marcantonio, P., B. Del Re, A. Franceschini, M. Capri, S. Lukas, F. Bersani, and G. Giorgi, "Synergic effect of retinoic acid and extremely low frequency magnetic field exposure on human neuroblastoma cell line BE(2) C," Bioelectromagnetics, Vol. 31, No. 6, 425-433, 2010.
doi:10.1002/bem.20581 Google Scholar

18. Whissell, P. D. and M. A. Persinger, "Emerging synergisms between drugs and physiologicallypatterned weak magnetic fields: Implications for neuropharmacology and the human population in the twenty-first century," Curr. Neuropharmacol., Vol. 5, No. 4, 278-288, 2007. Google Scholar

19. Abemayor, E. and N. Sidell, "Human neuroblastoma cell lines as models for the in vitro study of neoplastic and neuronal cell differentiation," Environ. Health Perspect., Vol. 80, No. 3, 1989. Google Scholar

20. Lanciotti, M., P. Montaldo, S. Folghera, E. Lucarelli, and P. Cornaglia-Ferraris, "A combined evaluation of biochemical and morphological changes during human neuroblastoma cell differentiation," Cell Mol. Neurobiol., Vol. 12, 225-240, 1992.
doi:10.1007/bf00712928 Google Scholar

21. Sidell, N., "Retinoic acid-induced growth inhibition and morphologic differentiation of human neuroblastoma cells in vitro," J. Natl. Cancer Inst., Vol. 68, 589-596, 1982. Google Scholar

22. Foletti, A., S. Grimaldi, A. Lisi, M. Ledda, and A. R. Liboff, "Bioelectromagnetic medicine: The role of resonance signaling," Electromagn. Biol. Med., Vol. 32, No. 4, 484-499, 2013.
doi:10.3109/15368378.2012.743908 Google Scholar

Alberto Foletti

Settimio Grimaldi

Mario Ledda

Antonella Lisi