Refractory gynecological infections are often very difficult to treat. The need for new strategies in their management is therefore continuously increasing. Some researchers have recently pointed up that microorganisms could emit specific electromagnetic signals. Moreover electromagnetic signals could be able to yield response from immune system. The aim of this study was to assess the possibility to employ electromagnetic signals from swabs of refractory gynecological infections to rise a response of the immune system able to erase them. Twenty-two consecutive patients with refractory gynecological infections, since at least 3 months, participate into this study employing an electro medical device (Med Matrix) to perform the electromagnetic information delivery procedure. Vaginal swabs was picked up for microbiological assessment at baseline and after 7 days. Out of 22 swabs performed, 15 were positive for microbiological assessment at baseline. Out of the 15 positive at baseline only 2 were still positive after one week of biophysical treatment (p < 0.0031). Biophysical treatment of refractory gynecological infections seems to be an useful and promising second line clinical tool.