paper 150122235539

An experiment to measure the effects of low frequency magnetic fields on cell prolif- eration was performed. Magnetic fields of low amplitudes, ranging from 1 to 4 mT, at frequencies of 60, 100, 800, 1500 and 2450 Hz were used to stimulate cell cultures of yeast Saccharomyces cerevisiae. The stimulation was carried out during 5 minutes, with intervals of 5 minutes rest, for 8 hours. A control and an experimental sample were measured two times for each frequency at the beginning of the experiment and at 2, 4, 6 and 8 hours later. In each measurement series a new cell culture was prepared for both non-stimulated and stimulated samples. The cells were grown in a liquid medium composed by 0.3 g yeast extract, 1.0 g gelatin peptone and 2.0 g dex- trose for 100 ml solution of growth medium, with pH adjusted to 4.5 with NaOH. Measurements of cell proliferation were based on light absorbance using a Beckman DU-650 spectrophotometer at 600 nm wavelength. A comparison of growth rate showed an increasing of cell proliferation in stimulated cells compared to the non-stimulated ones, with differences from 8% to 16% after 8 hours of exposure, for most of the frequencies employed, except at 60 and 800 Hz, where the control sample showed an increasing larger than the stimulated cultures, particularly at the final of the experiment. For the first hours of the experiments the results are variable, coming to present a clear trend only after 4 hours. In conclusion, the results of this experiment show an influence of magnetic fields on cell evolution, which is a function of both the exposure time and the stimulation frequency.

Citation

Modesto Sosa Aquino, Teodoro Cordova-Fraga, A. Horta-Rangel, A. Martinez-Longoria, R. Monroy-Torres, N. Padilla-Raygoza, M. Sabanero, and J. C. Villagomez, "Analysis of Light Absorbance on the Effects of Low Frequency Magnetic Fields on Cell Proliferation," Proceedings of 2015 Photonics & Electromagnetics Research Symposium, Prague, July 6 - 9，Page(s)1812-1814

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